|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21496||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepTransluc (Panomics)
- Backbone size w/o insert (bp) 4800
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name1.5kb 5' of miR-145
SpeciesH. sapiens (human)
Insert Size (bp)1500
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site Bgl II (not destroyed)
- 5′ sequencing primer ggtaccgagctcttacgcgt sequencing primer sequence (Common Sequencing Primers)
Plasmid 21496: P-miR-145 (pTransluc-miR-145-1) has a mutation in the miR-145 sequence resulting in A to G mutation. The mutation is a SNP from the human ES cells H9.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:P-miR-145 (pTransluc-miR-145-1) was a gift from Kenneth Kosik (Addgene plasmid # 21496 ; http://n2t.net/addgene:21496 ; RRID:Addgene_21496)
For your References section:MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells. Xu N, Papagiannakopoulos T, Pan G, Thomson JA, Kosik KS. Cell. 2009 May 15. 137(4):647-58. 10.1016/j.cell.2009.02.038 PubMed 19409607