psiCHECK-VIM270-gCM
(Plasmid #215868)

• Purpose: This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
• Depositing Lab: Kumiko Ui-Tei
• Publication: Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. doi: 10.1021/acsomega.7b00291. Epub 2017 May 12.

Backbone

• Vector backbone: psiCHECK-1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: target site which is completely complementary to guide strand of siRNA for vimentin is inserted to 3'UTR of RLuc
• Species: H. sapiens (human)
• Entrez Gene: VIM

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry