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Chemical Modification of the siRNA Seed Region Suppresses Off-Target Effects by Steric Hindrance to Base-Pairing with Targets.
Iribe H, Miyamoto K, Takahashi T, Kobayashi Y, Leo J, Aida M, Ui-Tei K
ACS Omega. 2017 May 31;2(5):2055-2064. doi: 10.1021/acsomega.7b00291. Epub 2017 May 12.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
215868psiCHECK-VIM270-gCMThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
215869psiCHECK-VIM270-pCMThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
215870psiCHECK-VIM270-gSMThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
215871psiCHECK-VIM270-pSMThis plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

Antibodies from Article