Efg1-ΔDBD-Y450S/Y452S/Y456S
(Plasmid
#216410)
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PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutations
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepJ411
- Backbone size w/o insert (bp) 4005
- Total vector size (bp) 5142
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEfg1-ΔDBD-Y450S/Y452S/Y456S
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SpeciesCandida albicans (Yeast)
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Insert Size (bp)1137
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Mutationchanged Tyrosine 450, 452 and 456 to Serine, and deleted amino acids 182-356
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Tag
/ Fusion Protein
- 6xHis-tags (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.09.566450 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Efg1-ΔDBD-Y450S/Y452S/Y456S was a gift from Nicolas Fawzi (Addgene plasmid # 216410 ; http://n2t.net/addgene:216410 ; RRID:Addgene_216410) -
For your References section:
Structure and position-specific interactions of prion-like domains in transcription factor Efg1 phase separation. Wang SH, Zheng T, Fawzi NL. bioRxiv [Preprint]. 2023 Nov 9:2023.11.09.566450. doi: 10.1101/2023.11.09.566450. 10.1101/2023.11.09.566450 PubMed 37986834
