Addgene: Structure and position-specific interactions of prion-like domains in transcription factor Efg1 phase separation.

This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Browse
Nicolas Fawzi
Wang et al

Structure and position-specific interactions of prion-like domains in transcription factor Efg1 phase separation.
Wang SH, Zheng T, Fawzi NL
bioRxiv [Preprint]. 2023 Nov 9:2023.11.09.566450. doi: 10.1101/2023.11.09.566450. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
216395	His-MBP-FL-Efg1	Expresses MBP-tagged full length Efg1
216396	Efg1-ΔDBD	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1
216397	Efg1-N-PrLD	Expresses 6xHis-tagged N-terminal prion-like domains of Efg1
216398	Efg1-C-PrLD	Expresses 6xHis-tagged C-terminal prion-like domains of Efg1
216399	Efg1-DBD	Expresses 6xHis-tagged DBD of Efg1
216400	Efg1-ΔDBD-A51P/A56P	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutations
216401	Efg1-ΔDBD-Q443P	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation
216402	Efg1-ΔDBD-A51P/A56P/Q443P	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations
216403	Efg1-ΔDBD-A51P/A56P/Q70P/Q443P	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations
216404	Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations
216405	Efg1-ΔDBD-Y4S/Y8S/Y16S	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutations
216406	Efg1-ΔDBD-Y64S/Y66S/Y69S	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutations
216407	Efg1-ΔDBD-Y101S/Y104S/Y106S	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutations
216409	Efg1-ΔDBD-Y401S/Y404S/Y409S	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutations
216410	Efg1-ΔDBD-Y450S/Y452S/Y456S	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutations
216411	Efg1-ΔDBD-Y478S/Y482S/Y484S	Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations

Antibodies from Article