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pIS1 DICER1 long-mut-let-7
(Plasmid #21651)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21651 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIS1
  • Backbone manufacturer
    Available from Addgene (#12179)
  • Backbone size w/o insert (bp) 4085
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dicer 1
  • Alt name
    CM10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • Mutation
    Mutant let-7 microRNA binding sites
  • Entrez Gene
    DICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2)
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer EBV Reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Long means that all functional alternative polyadenylation signals have been deleted.

Mut means that all microRNA binding sites for an indicated microRNA have been mutated.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIS1 DICER1 long-mut-let-7 was a gift from David Bartel (Addgene plasmid # 21651 ; http://n2t.net/addgene:21651 ; RRID:Addgene_21651)
  • For your References section:

    Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. Mayr C, Bartel DP. Cell. 2009 Aug 21. 138(4):673-84. 10.1016/j.cell.2009.06.016 PubMed 19703394