pIS1 DICER1 long UTR
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21649||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerAvailable from Addgene (#12179)
- Backbone size w/o insert (bp) 4085
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameDicer 1 long UTR
SpeciesH. sapiens (human)
Insert Size (bp)4200
Entrez GeneDICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2)
/ Fusion Protein
- Luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Xba I (not destroyed)
- 3′ cloning site Not I (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EBV Reverse (Common Sequencing Primers)
Long means that all functional alternative polyadenylation signals have been deleted.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIS1 DICER1 long UTR was a gift from David Bartel (Addgene plasmid # 21649 ; http://n2t.net/addgene:21649 ; RRID:Addgene_21649)
For your References section:Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. Mayr C, Bartel DP. Cell. 2009 Aug 21. 138(4):673-84. 10.1016/j.cell.2009.06.016 PubMed 19703394