pMSCV PIG IMP-1-long-mut
(Plasmid #21661)

• Depositing Lab: David Bartel
• Publication: Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Backbone

• Vector backbone: pMSCV PIG
• Backbone size w/o insert (bp): 7600
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IGF-II mRNA-binding protein 1
• Alt name: IMP-1
• Alt name: CM20
• Species: H. sapiens (human)
• Insert Size (bp): 8500
• Mutation: mutated let-7 binding sites.
• Entrez Gene: IGF2BP1 (a.k.a. CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1)
• Tags / Fusion Proteins:
  • IRES (C terminal on backbone)
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: pBABE 5
• 3′ sequencing primer: MSCV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Long means that all functional alternative polyadenylation signals have been deleted.

Mut means that all microRNA binding sites for an indicated microRNA have been mutated.

Constructs for Soft-agar assay