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PERK 1: PERK.WT.9E10.pCDNA
(Plasmid #21814)

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Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    We have found that the PERK cDNA confers instability (low replication ability) to plasmids. The plasmid will be provided in the STBL2 bacterial strain (gibco) which helps alleviate this problem. To recover the plasmid, stick a sterile toothpick into the stab and streak on an LB amp plate and grow at 30C. Pick an individual colony and grow in LB amp at 30C for the highest yield of plasmid.
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    PERK
  • Alt name
    Eif2ak3 eukaryotic translation initiation factor 2 alpha kinase 3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3577
  • Entrez Gene
    Eif2ak3 (a.k.a. AI427929, Pek, Perk)
  • Tag / Fusion Protein
    • Myc (9E10) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Mouse PERK, wild-type, 9E10-tagged at C-terminus, mammalian expression vector.

Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PERK 1: PERK.WT.9E10.pCDNA was a gift from David Ron (Addgene plasmid # 21814)