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Plasmid 21814: PERK 1: PERK.WT.9E10.pCDNA
  • PERK

  • Eif2ak3 eukaryotic translation initiation factor 2 alpha kinase 3

  • 3577

  • M. musculus (mouse)

  • Eif2ak3 (AI427929, Pek, Perk)

  • Myc (9E10)

  • C terminal on insert

  • pCDNA
    (Search Vector Database)

  • Mammalian Expression

  • 4756

  • EcoRI

  • No

  • XhoI

  • No

  • CMV-F List of Sequencing Primers

  • Ampicillin

  • Stbl2

  • 30

  • We have found that the PERK cDNA confers instability (low replication ability) to plasmids. The plasmid will be provided in the STBL2 bacterial strain (gibco) which helps alleviate this problem. To recover the plasmid, stick a sterile toothpick into the stab and streak on an LB amp plate and grow at 30C. Pick an individual colony and grow in LB amp at 30C for the highest yield of plasmid.

  • Low Copy

  • View sequences (2)
  • David Ron

  • UBMTA

Comments: 

Mouse PERK, wild-type, 9E10-tagged at C-terminus, mammalian expression vector.

Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21814" in your Materials and Methods section.