Addgene - IRE1 alpha-pcDNA3.EGFP Plasmid Data

Plasmid 13009: IRE1 alpha-pcDNA3.EGFP

Gene/insert name:
Inositol Requiring Enzyme-1
Alt name:
IRE1
Insert size:
3500
Species:
H. sapiens (human)
Entrez Gene:
ERN1 (IRE1, IRE1P, IRE1a, hIRE1p, FLJ30999, MGC163277, MGC163279)
Vector backbone:
pcDNA3-EGFP
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
Sp6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
None
Person or lab that originally
cloned the gene/insert:
cDNA is from Dr. Randy Kaufman at the University of Michigan. The vector is from Dr. David Ron at the New York University School of Medicine.
Sequence: View sequences (1)
Principal Investigator:
Fumihiko Urano
Terms and Licenses:
MTA

Comments:

NB: Does not contain an EGFP cassette

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IRE1. Lipson et al (Cell Metab. 2006 Sep . 4(3):245-54. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13009" in your Materials and Methods section.