9E10.mCHOP(S78&81A).pCDNA1/Amp
(Plasmid #21902)

• Depositing Lab: David Ron
• Publication: CHOP plasmids (unpublished)

Backbone

• Vector backbone: pcDNA1.1/Amp
• Backbone size w/o insert (bp): 4767
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CHOP
• Species: M. musculus (mouse)
• Insert Size (bp): 560
• Mutation: S78A S81A
• Entrez Gene: Ddit3 (a.k.a. AltDDIT3, CHOP-10, CHOP10, chop, gadd153)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

9E10 (Myc-epitope) tagged mouse CHOP coding region only, S78A and S81A double mutant. The S78A and S81A mutation introduces an additional HhaI site not present in the wildtype insert. Note that there is considerable uncertainity regarding this plasmid.