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pCherry-mNrp1
(Plasmid #21934)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pAcGFP1-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    neuropilin 1
  • Alt name
    Nrp; NP-1; Npn1; NPN-1; C530029I03; Nrp1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2769
  • GenBank ID
    NM_008737
  • Entrez Gene
    Nrp1 (a.k.a. C530029I03, NP-1, NPN-1, Npn1, Nrp)
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • Cherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer CMV_fwd_primer
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

AcGFP was substituted with Cherry from Roger Tsien's laboratory cloned between AgeI and NotI

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCherry-mNrp1 was a gift from Guido Serini (Addgene plasmid # 21934)
  • For your References section:

    Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. Valdembri D, Caswell PT, Anderson KI, Schwarz JP, Koenig I, Astanina E, Caccavari F, Norman JC, Humphries MJ, Bussolino F, Serini G. PLoS Biol. 2009 Jan 27. 7(1):e25. 10.1371/journal.pbio.1000025 PubMed 19175293