- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
Gene/Insert nameneuropilin 1
Alt nameNrp; NP-1; Npn1; NPN-1; C530029I03; Nrp1
SpeciesM. musculus (mouse)
Insert Size (bp)2769
Entrez GeneNrp1 (a.k.a. C530029I03, NP-1, NPN-1, Npn1, Nrp)
/ Fusion Proteins
- His (N terminal on insert)
- Cherry (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SacII (not destroyed)
- 5′ sequencing primer CMV_fwd_primer (Common Sequencing Primers)
AcGFP was substituted with Cherry from Roger Tsien's laboratory cloned between AgeI and NotI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCherry-mNrp1 was a gift from Guido Serini (Addgene plasmid # 21934)
For your References section:Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. Valdembri D, Caswell PT, Anderson KI, Schwarz JP, Koenig I, Astanina E, Caccavari F, Norman JC, Humphries MJ, Bussolino F, Serini G. PLoS Biol. 2009 Jan 27. 7(1):e25. 10.1371/journal.pbio.1000025 PubMed 19175293
Map generated by Addgene from plasmid data.