|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22034||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Alt namemVenus-LOV(Leu404-Leu546 , C450M)-Rac1(Ile4)-Q61L/E91H/N92H
SpeciesH. sapiens (human); Avena sativa (oat)
Insert Size (bp)1800
MutationThe LOV domain contains the mutation C450M.Rac1 starts at I4 and contains mutations Q61L, E91H and N92H.
Entrez GeneRAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pBabe-TetCMV Forward (Common Sequencing Primers)
Terms and Licenses
Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
Please note, Addgene has not confirmed all the mutations associated with this construct. We suggest sequence verifying this plasmid upon receipt.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M was a gift from Klaus Hahn (Addgene plasmid # 22034 ; http://n2t.net/addgene:22034 ; RRID:Addgene_22034)
For your References section:A genetically encoded photoactivatable Rac controls the motility of living cells. Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM. Nature. 2009 Sep 3. 461(7260):104-8. 10.1038/nature08241 PubMed 19693014