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pBabe-TetCMV-puro-mCherry-PA-Rac1
(Plasmid #22035)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22035 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-TetCMV-puro
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-PA-Rac1
  • Alt name
    mCherry-LOV(Leu404-Leu546)-Rac1(Ile4)-Q61L/E91H/N92H
  • Species
    H. sapiens (human); Avena sativa (oat)
  • Insert Size (bp)
    1800
  • Mutation
    Rac1 starts at I4 and contains mutations Q61L, E91H and N92H.
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pBabe-TetCMV Forward
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Protocols for production and use of Hahn lab biosensors and photoactivatable proteins can be found on their web page, at the URL below. If you have suggestions for the protocols or have any questions, please feel free to contact the Hahn lab. Good luck with your experiments!

Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-TetCMV-puro-mCherry-PA-Rac1 was a gift from Klaus Hahn (Addgene plasmid # 22035)
  • For your References section:

    A genetically encoded photoactivatable Rac controls the motility of living cells. Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM. Nature. 2009 Sep 3. 461(7260):104-8. 10.1038/nature08241 PubMed 19693014