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pBabe-TetCMV-puro-mCherry-PA-Rac1 Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pBabe-TetCMV-puro-mCherry-PA-Rac1 with the following enzymes: EcoRI-HF and XhoI.

We predict the following pattern:
EcoRI-HF - 7.6 kb
XhoI - 7.6 kb
EcoRI-HF + XhoI - 5.8 and 1.8 kb

We see the expected result