Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22088||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
Copy numberHigh Copy
Alt nameFGF8 splice variant 4(A)
SpeciesM. musculus (mouse)
Insert Size (bp)840
Entrez GeneFgf8 (a.k.a. Ai, Aigf, Fgf-8)
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Mouse FGF8 cDNA splice variant 4(A) clone was isolated from an E7.5 cDNA library. The insert contains the entire coding region and 3' UTR. The SmaI site is in the FGF8 5'UTR 16bp upstream of the translation start site. The 3' XhoI site is from the vector MCS. The FGF8 variant 4(A) sequence is ~30bp shorter than the variant 1(B) sequence.
For antisense probe linearize with BamHI and use T7 Polymerase.The transcript is ~840bp.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mFGF8a was a gift from Gail Martin (Addgene plasmid # 22088 ; http://n2t.net/addgene:22088 ; RRID:Addgene_22088)
For your References section:The mouse Fgf8 gene encodes a family of polypeptides and is expressed in regions that direct outgrowth and patterning in the developing embryo. Crossley PH, Martin GR. Development. 1995 Feb . 121(2):439-51. PubMed 7768185
Map uploaded by the depositor.