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shHIF2α(#2) (HIF2α-#2-pSUPER-retro-GFP/Neo)
(Plasmid #22131)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22131 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSUPER_Retro-GFP/Neo
  • Backbone manufacturer
    OligoEngine
  • Backbone size w/o insert (bp) 7394
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a or HB101
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    synthethic oligonucleotide
  • Insert Size (bp)
    58
  • Mutation
    synthethic oligonucleotide encoding shRNA targeting human HIF2alpha mRNA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (destroyed during cloning)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer MSCV reverse
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

shRNA oligo sequence - 5'-GATCCCCGACAAGGTCTGCAAAGGGTTTCAAGAGAACCCTTTGCAGACCTTGTCTTTTT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    shHIF2α(#2) (HIF2α-#2-pSUPER-retro-GFP/Neo) was a gift from William Kaelin (Addgene plasmid # 22131 ; http://n2t.net/addgene:22131 ; RRID:Addgene_22131)
  • For your References section:

    Hypoxia-inducible factor linked to differential kidney cancer risk seen with type 2A and type 2B VHL mutations. Li L, Zhang L, Zhang X, Yan Q, Minamishima YA, Olumi AF, Mao M, Bartz S, Kaelin WG. Mol Cell Biol. 2007 Aug . 27(15):5381-92. 10.1128/MCB.00282-07 PubMed 17526729