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Plasmid 22255: pLenti CMV GSE T329A Hygro (w189-4)
  • Genetic suppressor element #22, T329A

  • GS22 T329

  • 245

  • R. norvegicus (rat)

  • Threonine 329 is mutated to Alanine (p53 nomencature).

  • p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 8712

  • Bam HI

  • No

  • Xba I

  • No

  • CMVforw List of Sequencing Primers

  • n/a

  • Ampicillin

  • Stbl3

  • 37

  • Stbl3, 37oC

  • High Copy

  • Hygromycin

  • GSE22 T329A was obtained from Dr. Christian Beausejour.

  • View sequences (2)
  • View map

  • Eric Campeau

  • UBMTA
    Ancillary Agreement for Plasmids Containing FP Materials

Comments: 

Encodes amino acids #312-391 of rat p53 to act as a dominant-negative
(Ossovskaya et al. 1996, PNAS 93:10309-10314).

T329A mutation does not prevent tetramerization of p53
(Mateu et al. Nat Struct Biol. 1999 Feb;6(2):191-8).

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22255" in your Materials and Methods section.