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pLenti CMV GSE T329A Hygro (w189-4)
(Plasmid #22255)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22255 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size w/o insert (bp) 8712
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3, 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Genetic suppressor element #22, T329A
  • Alt name
    GS22 T329
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    245
  • Mutation
    Threonine 329 is mutated to Alanine (p53 nomencature).

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer CMVforw
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GSE22 T329A was obtained from Dr. Christian Beausejour.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encodes amino acids #312-391 of rat p53 to act as a dominant-negative
(Ossovskaya et al. 1996, PNAS 93:10309-10314).

T329A mutation does not prevent tetramerization of p53
(Mateu et al. Nat Struct Biol. 1999 Feb;6(2):191-8).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMV GSE T329A Hygro (w189-4) was a gift from Eric Campeau (Addgene plasmid # 22255 ; http://n2t.net/addgene:22255 ; RRID:Addgene_22255)