Addgene - pLenti CMV GSE T329A Hygro (w189-4) Plasmid Data

Plasmid 22255: pLenti CMV GSE T329A Hygro (w189-4)

Gene/insert name:
Genetic suppressor element #22, T329A
Alt name:
GS22 T329
Insert size:
245
Species:
R. norvegicus (rat)
Mutation:
Threonine 329 is mutated to Alanine (p53 nomencature).
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
8712
Cloning site 5':
Bam HI
Site destroyed during cloning:
No
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
CMVforw List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC
High or low copy:
High Copy
Selectable markers:
Hygromycin
Person or lab that originally
cloned the gene/insert:
GSE22 T329A was obtained from Dr. Christian Beausejour.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

Encodes amino acids #312-391 of rat p53 to act as a dominant-negative
(Ossovskaya et al. 1996, PNAS 93:10309-10314).

T329A mutation does not prevent tetramerization of p53
(Mateu et al. Nat Struct Biol. 1999 Feb;6(2):191-8).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22255" in your Materials and Methods section.