Addgene - TetR in pENTR1A (559-1) Plasmid Data

Plasmid 22265: TetR in pENTR1A (559-1)

Gene/insert name:
Tetracycline repressor
Alt name:
TetR
Insert size:
1303
Entrez Gene:
tetR (SMR0223)
Vector backbone:
pENTR1A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Yeast Expression, Worm Expression, Insect Expression
Backbone size w/o insert (bp):
2386
Cloning site 5':
Kpn I
Site destroyed during cloning:
No
Cloning site 3':
Eco RV
Site destroyed during cloning:
Yes
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
TetR cDNA was derived from the pLenti 6/TR plasmid (Invitrogen).
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA
Tet Systems Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22265" in your Materials and Methods section.