TetR in pENTR1A (559-1)
- Backbone size w/o insert (bp) 2386
Vector typeMammalian Expression, Yeast Expression, Worm Expression, Insect Expression
Growth in Bacteria
Growth instructionsTOP10F', 37oC
Copy numberHigh Copy
Gene/Insert nameTetracycline repressor
Insert Size (bp)1303
Entrez GenetetR (a.k.a. SMR0223)
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn I (not destroyed)
- 3′ cloning site Eco RV (destroyed during cloning)
- 5′ sequencing primer ENTRforw
- 3′ sequencing primer ENTRrev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byTetR cDNA was derived from the pLenti 6/TR plasmid (Invitrogen).
Terms and Licenses
Please note that there are several sequence discrepancies between Addgene's quality control sequence and the depositor's reference sequence. These changes do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TetR in pENTR1A (559-1) was a gift from Eric Campeau (Addgene plasmid # 22265)
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.