TetR in pENTR1A (559-1)
(Plasmid #22265)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pENTR1A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2386
  • Vector type
    Mammalian Expression, Yeast Expression, Worm Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10F', 37oC
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Tetracycline repressor
  • Alt name
    TetR
  • Insert Size (bp)
    1303
  • Entrez Gene
    tetR (a.k.a. SMR0223)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site Eco RV (destroyed during cloning)
  • 5′ sequencing primer ENTRforw
  • 3′ sequencing primer ENTRrev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    TetR cDNA was derived from the pLenti 6/TR plasmid (Invitrogen).
  • Terms and Licenses

Depositor Comments

Please note that there are several sequence discrepancies between Addgene's quality control sequence and the depositor's reference sequence. These changes do not affect plasmid function.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TetR in pENTR1A (559-1) was a gift from Eric Campeau (Addgene plasmid # 22265)