|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22280||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4864
Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
SpeciesA. thaliana (mustard weed)
Insert Size (bp)1023
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site Not1 (destroyed during cloning)
- 5′ sequencing primer SV40 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAL197 was a gift from Christopher Voigt (Addgene plasmid # 22280)
For your References section:Spatiotemporal control of cell signalling using a light-switchable protein interaction. Levskaya A, Weiner OD, Lim WA, Voigt CA. Nature. 2009 Sep 13. ():. 10.1038/nature08446 PubMed 19749742