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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneRSV-SG
- Backbone size w/o insert (bp) 3600
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCREB
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1200
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Entrez GeneCreb1 (a.k.a. Creb)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba 1 (destroyed during cloning)
- 3′ cloning site Bal1 (destroyed during cloning)
- 5′ sequencing primer RSV (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
insert can be released by digesting with HindIII/Xba1
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RSV CREB was a gift from Marc Montminy (Addgene plasmid # 22394 ; http://n2t.net/addgene:22394 ; RRID:Addgene_22394) -
For your References section:
Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133. Gonzalez GA, Montminy MR. Cell. 1989 Nov 17. 59(4):675-80. 10.1016/0092-8674(89)90013-5 PubMed 2573431
Map uploaded by the depositor.
