Addgene - p3Eegfp Plasmid Data

Plasmid 22462: p3Eegfp

Gene/insert name:
EGFP
Insert size:
700
Entrez Gene:
eGFP (pPRS3a_01)
Vector backbone:
pDONRP2r-P3
(Search Vector Database)
Vector type:
Entry Vector
Backbone size w/o insert (bp):
3000
Cloning method:
Gateway Cloning
5' sequencing primer:
M13_puc_F List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Nathan Lawson
Terms and Licenses:
MTA

Comments:

This clone was amplified with an attB2 site, at the 5’ end and a attB3 site on the 3’ end. The product was then recombined with the pDNR P2R-P3 vector.

Can be used to make 3’ end (C-Term) tagged fusion construct.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc et al (Dev Dyn. 2007 Nov . 236(11):3077-87. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22462" in your Materials and Methods section.