Addgene - Flag-HA-USP43 Plasmid Data

Plasmid 22583: Flag-HA-USP43

Gene/insert name:
USP43
Alt name:
ubiquitin specific peptidase 43
Alt name:
FLJ30626
Species:
H. sapiens (human)
GenBank ID:
NM_153210
Entrez Gene:
USP43 (FLJ30626)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Mutation:
not FL, missing first 108 aa
Vector backbone:
pDEST_LTR_N_FLAG_HA_IRES_puro
(Search Vector Database)
Backbone manufacturer:
Harper_Lab
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6521
Cloning method:
Gateway Cloning
Cloning site 5':
GATEWAY_LR
Site destroyed during cloning:
Unknown
Cloning site 3':
GATEWAY_LR
Site destroyed during cloning:
Unknown
5' sequencing primer:
CAGCCCTCACTCCTTCTCTAGG List of Sequencing Primers
3' sequencing primer:
CAAGCGGCTTCGGCCAGTAAC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Wade Harper
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Defining the human deubiquitinating enzyme interaction landscape. Sowa et al (Cell. 2009 Jul 23. 138(2):389-403. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22583" in your Materials and Methods section.