Addgene - Flag-HA-USP45 Plasmid Data

Plasmid 22605: Flag-HA-USP45

Gene/insert name:
USP45
Alt name:
ubiquitin specific peptidase 45
Alt name:
MGC14793
Species:
H. sapiens (human)
GenBank ID:
NM_001080481
Entrez Gene:
USP45 (MGC14793)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Mutation:
None
Vector backbone:
pDEST_Tet_ON_CMV_N_FLAG_HA_PGK_puro
(Search Vector Database)
Backbone manufacturer:
Harper_Lab
Vector type:
Mammalian Expression, Retroviral
Cloning method:
Gateway Cloning
Cloning site 5':
GATEWAY_LR
Site destroyed during cloning:
Unknown
Cloning site 3':
GATEWAY_LR
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV_forward List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Wade Harper
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Defining the human deubiquitinating enzyme interaction landscape. Sowa et al (Cell. 2009 Jul 23. 138(2):389-403. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22605" in your Materials and Methods section.