|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22677||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeMouse Targeting
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameDTA and TK
Insert Size (bp)500
MutationpDTA-TK dual selection plasmid for targeting vector generation. I-CeuI linearization site.
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDTA-TK was a gift from Robert Benezra (Addgene plasmid # 22677 ; http://n2t.net/addgene:22677 ; RRID:Addgene_22677)
For your References section:High levels of Id1 expression define B1 type adult neural stem cells. Nam HS, Benezra R. Cell Stem Cell. 2009 Nov 6. 5(5):515-26. 10.1016/j.stem.2009.08.017 PubMed 19896442