Addgene - pISI-mmP16 3' UTR Plasmid Data

Plasmid 22693: pISI-mmP16 3' UTR

Gene/insert name:
mmP16 3'UTR
Alt name:
luciferase reporter
Insert size:
2000
Species:
Renilla
Vector backbone:
pIS1
(Search Vector Database)
Backbone manufacturer:
Bartel lab (Addgene#:12179)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4085
Cloning site 5':
SacI
Site destroyed during cloning:
Unknown
Cloning site 3':
AgeI
Site destroyed during cloning:
Unknown
5' sequencing primer:
Rluc-F List of Sequencing Primers
3' sequencing primer:
EBV-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

-3'UTR segment cloned from HMLE sDNA

-2000bps of the MMP16 3'UTR downstream of a renilla luciferase reporter gene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. Valastyan et al (Cell. 2009 Jun 12. 137(6):1032-46. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22693" in your Materials and Methods section.