Addgene - pMCS.71/2272 Plasmid Data

Plasmid 22732: pMCS.71/2272

Gene/insert name:
Lox71-MCS-Lox2272
Insert size:
150
Mutation:
This is a basal exchange vector for RMCE containing heterotypic LoxP sites. A lox71 site, flanked by Sac I and Not I, and a lox2272 site, flanked by Xho I and Kpn I, were inserted into the same sites of pBluescript KS+.
Vector backbone:
pBluescript KS+
(Search Vector Database)
Vector type:
Cre/Lox
Backbone size w/o insert (bp):
2905
Cloning site 5':
Sac1
Site destroyed during cloning:
No
Cloning site 3':
Kpn1
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5alfa, XL1-blue, or other competent bacterial cell line
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Mark Magnuson
Terms and Licenses:
MTA

Comments:

Please note that the plasmid names and lox sites have been corrected since publication.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Chen et al (Dis Model Mech. 2011 Feb 14. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22732" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

22731: pMCS.71/2272.Hygro