|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22732||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS+
- Backbone size w/o insert (bp) 2905
Growth in Bacteria
Growth instructionsDH5alfa, XL1-blue, or other competent bacterial cell line
Copy numberHigh Copy
Insert Size (bp)150
MutationThis is a basal exchange vector for RMCE containing heterotypic LoxP sites. A lox71 site, flanked by Sac I and Not I, and a lox2272 site, flanked by Xho I and Kpn I, were inserted into the same sites of pBluescript KS+.
- Cloning method Restriction Enzyme
- 5′ cloning site Sac1 (not destroyed)
- 3′ cloning site Kpn1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid names and lox sites have been corrected since publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMCS.71/2272 was a gift from Mark Magnuson (Addgene plasmid # 22732 ; http://n2t.net/addgene:22732 ; RRID:Addgene_22732)
For your References section:Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Chen SX, Osipovich AB, Ustione A, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA. Dis Model Mech. 2011 Feb 14. ():. 10.1242/dmm.006569 PubMed 21324933