Addgene - pAdTrack-CMV HA ALAS-1 Plasmid Data

Plasmid 22746: pAdTrack-CMV HA ALAS-1

Gene/insert name:
ALAS-1
Alt name:
ALAS1
Species:
M. musculus (mouse)
Entrez Gene:
Alas1 (ALAS, ALAS-N, Alas-1, Alas-h)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pAdTrack-CMV
(Search Vector Database)
Backbone manufacturer:
Available at Addgene (#16405)
Vector type:
Mammalian Expression, Adenoviral
Backbone size w/o insert (bp):
9000
Cloning site 5':
KpnI
Site destroyed during cloning:
Unknown
Cloning site 3':
NotI
Site destroyed during cloning:
Unknown
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (1)
Principal Investigator:
Bruce Spiegelman
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: PGC-1alpha negatively regulates hepatic FGF21 expression by modulating the heme/Rev-Erb(alpha) axis. Estall et al (Proc Natl Acad Sci U S A. 2009 Dec 29. 106(52):22510-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22746" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

16400: pAdEasy-1

16398: BJ5183 cells

9053: pSG5 Large T