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pMH4-SYN-ChR2-tdimer2RFP
(Plasmid #22772)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22772 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha, JM109 high efficiency
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ChR2-tdimer2RFP
  • Species
    mixture
  • Insert Size (bp)
    2330
  • Tag / Fusion Protein
    • ChR2-tdimer2RFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SAlI (not destroyed)
  • 5′ sequencing primer gactcagcgctgcctcagtctg
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

ChR2 seq.5'-3' 870-1796=926bp;
tdimer2RFP 5'-3' 1809-3200=1391bp

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH4-SYN-ChR2-tdimer2RFP was a gift from Thomas Oertner (Addgene plasmid # 22772)
  • For your References section:

    Optical induction of plasticity at single synapses reveals input-specific accumulation of alphaCaMKII. Zhang YP, Holbro N, Oertner TG. Proc Natl Acad Sci U S A. 2008 Aug 19. 105(33):12039-44. 10.1073/pnas.0802940105 PubMed 18697934