Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22780||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)720
MutationControl for intermolecular Rac1 biosensor FRET. Similar to pEGFP-C1, which was recloned to have Ypet.
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site Bgl II (not destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYpet-C1 was a gift from Klaus Hahn (Addgene plasmid # 22780 ; http://n2t.net/addgene:22780 ; RRID:Addgene_22780)
For your References section:Coordination of Rho GTPase activities during cell protrusion. Machacek M, Hodgson L, Welch C, Elliott H, Pertz O, Nalbant P, Abell A, Johnson GL, Hahn KM, Danuser G. Nature. 2009 Sep 3. 461(7260):99-103. 10.1038/nature08242 PubMed 19693013