Addgene - pYpet-PBD Plasmid Data

Plasmid 22781: pYpet-PBD

Gene/insert name:
PAK binding domain
Insert size:
300
Species:
H. sapiens (human)
Fusion protein or tag:
Ypet
Terminal:
N terminal on backbone
Vector backbone:
pYpet-C1
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
CMV, 3' XFP primer List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Klaus Hahn
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Coordination of Rho GTPase activities during cell protrusion. Machacek et al (Nature. 2009 Sep 3. 461(7260):99-103. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22781" in your Materials and Methods section.