Addgene - Myc tag WT PTB Plasmid Data

Plasmid 23024: Myc tag WT PTB

Gene/insert name:
PTB1
Alt name:
PTB
Species:
H. sapiens (human)
Entrez Gene:
PTBP1 (HNRNP-I, HNRNPI, HNRPI, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB)
Fusion protein or tag:
Myc
Terminal:
N terminal on insert
Vector backbone:
pCMV-Myc
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3800
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Douglas Black
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Protein kinase A phosphorylation modulates transport of the polypyrimidine tract-binding protein. Xie et al (Proc Natl Acad Sci U S A. 2003 Jul 22. 100(15):8776-81. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 23024" in your Materials and Methods section.