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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 23047 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGL3-control
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5223
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep27
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Alt namekip1
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SpeciesH. sapiens (human)
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Insert Size (bp)607
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Mutationwt cDNA
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GenBank IDU10906
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Entrez GeneCDKN1B (a.k.a. CDKN4, KIP1, MEN1B, MEN4, P27KIP1)
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Tag
/ Fusion Protein
- Luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Nco I (not destroyed)
- 5′ sequencing primer GLprimer2 (Promega) (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-p27Luc was a gift from William Kaelin (Addgene plasmid # 23047 ; http://n2t.net/addgene:23047 ; RRID:Addgene_23047) -
For your References section:
Bioluminescent imaging of Cdk2 inhibition in vivo. Zhang GJ, Safran M, Wei W, Sorensen E, Lassota P, Zhelev N, Neuberg DS, Shapiro G, Kaelin WG. Nat Med. 2004 Jun . 10(6):643-8. 10.1038/nm1047 PubMed 15122251