pRR-AR-5Z
(Plasmid #23058)

• Depositing Lab: Charles Miller
• Publication: Miller et al Plasmid. 2009 Dec 3. ():.

Backbone

• Vector backbone: pRW95-3
• Backbone manufacturer: PMID: 8800671
• Backbone size w/o insert (bp): 8700
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AR
• Alt name: androgen receptor
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SnaBI (destroyed during cloning)
• 3′ cloning site: SnaBI (destroyed during cloning)
• 5′ sequencing primer: GPDpro-F

Resource Information

• Supplemental Documents:
  • pRR-AR-5Z assay
• A portion of this plasmid was derived from a plasmid made by: The cDNA source for this plasmid was obtained from Aaron Caplan.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Amino acid G8 is missing, BLAST shows different number of Qs in sequence.

Please note that this construct was tested by the depositing lab. Dr Miller transformed yeast strain W303a with Addgene's plasmid and with his stock of the same plasmid. He also streaked out yeast that were previously transformed with this plasmid and validated for signaling of the lacZ reporter gene in response to testosterone.

The plasmids transformed well and conferred reporter gene signaling in the transformants when exposed to 25 nM testosterone. This bioassay indicates that the human androgen receptor DNA sequence produces a functional protein.