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pRR-ERalpha-5Z
(Plasmid #23061)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23061 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRW95-3
  • Backbone manufacturer
    PMID: 8800671
  • Backbone size w/o insert (bp) 8700
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERalpha
  • Alt name
    estrogen receptor alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Entrez Gene
    ESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer GAL1
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cDNA used to create this plasmid was obtained from Keith R. Yamamoto.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

There is a M517T mutation in the ESR1 sequence. The mutation should not have any effect on protein function and this construct worked the same when assessed in yeast signaling assays and estrogen signaling.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRR-ERalpha-5Z was a gift from Charles Miller (Addgene plasmid # 23061 ; http://n2t.net/addgene:23061 ; RRID:Addgene_23061)
  • For your References section:

    Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Miller CA, Tan X, Wilson M, Bhattacharyya S, Ludwig S. Plasmid. 2009 Dec 3. ():. 10.1016/j.plasmid.2009.11.003 PubMed 19962400