Addgene - pMOTag43M-crelox-PUR Plasmid Data

Plasmid 24032: pMOTag43M-crelox-PUR

Gene/insert name:
None
Fusion protein or tag:
3XHA
Terminal:
N terminal on backbone
Vector backbone:
pMOTag
(Search Vector Database)
Vector type:
Cre/Lox
Backbone size (bp):
5099
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
George Cross
Terms and Licenses:
MTA

Comments:

pMOTag43M-crelox-PUR made by Jenny Li taking the HindIII-BamHI crelox-PUR fragment from pyrFEKO-PUR and inserting into HindIII-BamHI digested pMOTag43M.

Based on pMOTag series made in pBluescript II KS+

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24032" in your Materials and Methods section.