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RPL3-GFP (96)
(Plasmid #24040)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24040 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    yEGFP3
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RPL3
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RPL3 (a.k.a. YOR063W, MAK8, TCM1, YOR29-14)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Entire RPL3 ORF inserted upstream of eGFP in EcoRI/HindIII of yEGFP3.

An F379S mutation found in Addgene quality control sequencing. No evidence to suggest mutation affects activity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RPL3-GFP (96) was a gift from Michael Rout (Addgene plasmid # 24040 ; http://n2t.net/addgene:24040 ; RRID:Addgene_24040)