pK.myc-MYPT1
(Plasmid #24101)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pKmyc
  • Backbone manufacturer
    Addgene plasmid 19400
  • Backbone size w/o insert (bp) 4200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Myosin-targeting subunit-1
  • Alt name
    MBS
  • Alt name
    MYPT1
  • Alt name
    M110
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Mutation
    S50G
  • GenBank ID
    AF458589
  • Entrez Gene
    PPP1R12A (a.k.a. M130, MBS, MYPT1)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK.myc-MYPT1 was a gift from Masumi Eto (Addgene plasmid # 24101)
  • For your References section:

    Assembly of MYPT1 with protein phosphatase-1 in fibroblasts redirects localization and reorganizes the actin cytoskeleton. Eto M, Kirkbride JA, Brautigan DL. Cell Motil Cytoskeleton. 2005 Oct . 62(2):100-9. 10.1002/cm.20088 PubMed 16106448