Addgene - pK.myc-MYPT1 Plasmid Data

Plasmid 24101: pK.myc-MYPT1

Gene/insert name:
Myosin-targeting subunit-1
Alt name:
MBS
Alt name:
MYPT1
Alt name:
M110
Insert size:
3000
Species:
H. sapiens (human)
GenBank ID:
AF458589
Entrez Gene:
PPP1R12A (M130, MBS, MYPT1)
Fusion protein or tag:
Myc
Terminal:
N terminal on backbone
Mutation:
S50G
Vector backbone:
pKmyc
(Search Vector Database)
Backbone manufacturer:
Addgene plasmid 19400
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4200
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Masumi Eto
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Assembly of MYPT1 with protein phosphatase-1 in fibroblasts redirects localization and reorganizes the actin cytoskeleton. Eto et al (Cell Motil Cytoskeleton. 2005 Oct . 62(2):100-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24101" in your Materials and Methods section.