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HeLa full-length MLCK-GFP
(Plasmid #46316)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46316 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 10500
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HeLa long myosin light chain kinase
  • Alt name
    human full-length MLCK 210
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5800
  • GenBank ID
    NC_000003.11
  • Entrez Gene
    MYLK (a.k.a. AAT7, KRP, MLCK, MLCK1, MLCK108, MLCK210, MMIHS, MSTP083, MYLK1, smMLCK)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HeLa full-length MLCK-GFP was a gift from Anne Bresnick (Addgene plasmid # 46316 ; http://n2t.net/addgene:46316 ; RRID:Addgene_46316)
  • For your References section:

    The N-terminus of the long MLCK induces a disruption in normal spindle morphology and metaphase arrest. Dulyaninova NG, Patskovsky YV, Bresnick AR. J Cell Sci. 2004 Mar 15;117(Pt 8):1481-93. 10.1242/jcs.00993 PubMed 15020676