Addgene - p3xFLAG-CMV10-hFIP200 Plasmid Data

Plasmid 24300: p3xFLAG-CMV10-hFIP200

Gene/insert name:
FIP200
Alt name:
focal adhesion kinase family interacting protein of 200 kD
Alt name:
RB1CC1
Alt name:
Rb1-inducible coiled coil protein 1
Insert size:
4785
Species:
H. sapiens (human)
GenBank ID:
BC017556
Entrez Gene:
RB1CC1 (CC1, FIP200)
Fusion protein or tag:
3xFLAG
Terminal:
N terminal on backbone
Vector backbone:
p3xFLAG-CMV-10
(Search Vector Database)
Backbone manufacturer:
sigma-aldrich
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6299
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
CMV 30 sequencing primer List of Sequencing Primers
3' sequencing primer:
CMV 24 sequencing primer
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
(cDNA clone MGC:9519, IMAGE:3908134)
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Noboru Mizushima
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells. Hara et al (J Cell Biol. 2008 May 5. 181(3):497-510. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24300" in your Materials and Methods section.