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7TF
(Plasmid #24306)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24306 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRRLSIN.cPPT.PGK-GFP.WPRE
  • Backbone manufacturer
    Naldini/Trono (Addgene #12252)
  • Backbone size w/o insert (bp) 6132
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    7xTcf-FFluc
  • Alt name
    FFluc
  • Alt name
    firefly luciferase
  • Alt name
    luciferase
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1919

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GGCCCGAAGGAATAGAAG
  • 3′ sequencing primer AACGGGCCACAACTCCTC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The 7xTcf promoter is derived from pSuperTOPflash, published in Veeman MT et al., Curr Biol 13: 680–685 (2003)
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

TFC repeat sequence is AGATCAAAGGgg

This plasmid requires a 2nd generation lentiviral packaging system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    7TF was a gift from Roel Nusse (Addgene plasmid # 24306 ; http://n2t.net/addgene:24306 ; RRID:Addgene_24306)
  • For your References section:

    Lentiviral vectors to probe and manipulate the wnt signaling pathway. Fuerer C, Nusse R. PLoS One. 2010 . 5(2):e9370. 10.1371/journal.pone.0009370 PubMed 20186325