Addgene - 7TGP Plasmid Data

Plasmid 24305: 7TGP

Gene/insert name:
7xTcf-eGFP // SV40-PuroR
Alt name:
GFP
Alt name:
puromycin N-acetyltransferase
Alt name:
enhanced GFP
Insert size:
2094
Species:
Aequorea victoria (modified) / Streptomyces alboniger
Mutation:
EGFP is GFP with S65T and F64L mutations
Vector backbone:
SIN lentivirus (cPPT+ WPRE+)
(Search Vector Database)
Vector type:
Lentiviral
Backbone size w/o insert (bp):
6132
Cloning site 5':
PstI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
GGCCCGAAGGAATAGAAG List of Sequencing Primers
3' sequencing primer:
AACGGGCCACAACTCCTC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
The 7xTcf promoter is derived from pSuperTOPflash, published in Veeman MT et al., Curr Biol 13: 680–685 (2003)
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Roel Nusse
Terms and Licenses:
MTA

Comments:

TCF repeat sequence is AGATCAAAGGgg.

This plasmid requires a 2nd generation lentiviral packaging system.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Lentiviral vectors to probe and manipulate the wnt signaling pathway. Fuerer et al (PLoS One. 2010 . 5(2):e9370. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24305" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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