Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

E[beta]P
(Plasmid #24313)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24313 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRRLSIN.cPPT.PGK-GFP.WPRE
  • Backbone manufacturer
    Naldini/Trono (Addgene #12252)
  • Backbone size w/o insert (bp) 6074
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EF1alpha-betacatenin(deltaGSK) // SV40-PuroR
  • Alt name
    betacat
  • Alt name
    beta-catenin
  • Alt name
    puromycin N-acetyltransferase
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4718
  • Mutation
    Sites phosphorylated by GSK-3beta have been mutated. S33A, S37A, T41A, S45A
  • GenBank ID
    NM_007614
  • Entrez Gene
    Ctnnb1 (a.k.a. Bfc, Cat, Catnb, Mesc)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (vec) and AvaII (insert) (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GGCCCGAAGGAATAGAAG
  • 3′ sequencing primer AACGGGCCACAACTCCTC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Beta-catenin(∆GSK) was obtained from Amanda Mikels)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

This plasmid requires a 2nd generation lentiviral packaging system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E[beta]P was a gift from Roel Nusse (Addgene plasmid # 24313 ; http://n2t.net/addgene:24313 ; RRID:Addgene_24313)
  • For your References section:

    Lentiviral vectors to probe and manipulate the wnt signaling pathway. Fuerer C, Nusse R. PLoS One. 2010 . 5(2):e9370. 10.1371/journal.pone.0009370 PubMed 20186325