|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24337||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4283
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site NcoI (unknown if destroyed)
- 5′ sequencing primer pCasper-hs (Common Sequencing Primers)
The XhoI/NcoI fragment containing QUAS-Pmin was subcloned from pQUAS-GG
into pGL4.23 (Promega) to replace its minimal promoter. pGL4.23 contains synthetic firefly
luciferase gene (luc2), which has been codon optimized for high expression in mammalian cells.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQUAS-luc2 was a gift from Liqun Luo (Addgene plasmid # 24337 ; http://n2t.net/addgene:24337 ; RRID:Addgene_24337)
For your References section:The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990