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pQUAS-GG
(Plasmid #24336)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 24336 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript SK(+)
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5xQUAS/GFP split with an intron (GG)
  • Species
    Neurospora crassa/other
  • Insert Size (bp)
    1500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid contains 5 copies of naturally occurring QF binding sites (each 16 bp
long, shown in capital letters, with spacer sequences in small letters):
GGGTAATCGCTTATCCtcGGATAAACAATTATCCtcacGGGTAATCGCTTATCCgctcGGGTAATCG
CTTATCCtcGGGTAATCGCTTATCCtt
This sequence was assembled from overlapping primers and cloned using XhoI and HindIII into
pBluescript containing hsp70 minimal promoter (Brand and Perrimon, 1993) and an optimized GFP
split with an intron (GG) (Zong et al., 2005)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQUAS-GG was a gift from Liqun Luo (Addgene plasmid # 24336 ; http://n2t.net/addgene:24336 ; RRID:Addgene_24336)
  • For your References section:

    The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990