|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24345||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5493
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)2600
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
GAL4 cDNA was PCR amplified using primers PR509
(CCCCGGATCCCAACatgaagctactgtcttctatcgaaca) and PR510
(CGGTTAACGCGGCCGCttactctttttttgggtttggtg), and a lab vector, pCA-GAL4 as the template. The
PCR product was cloned using BamHI and NotI into pCDNA3.1-myc-His-A.
A stop codon precedes the Myc and His tags.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-GAL4 was a gift from Liqun Luo (Addgene plasmid # 24345 ; http://n2t.net/addgene:24345 ; RRID:Addgene_24345)
For your References section:The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990