|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24342||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK(+)
- Backbone size (bp) 3000
Modifications to backboneGFP split with an intron (GG)
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- 5XGAL4 (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BglII (unknown if destroyed)
- 5′ sequencing primer GFP-N (Common Sequencing Primers)
The BamHI/BglI fragment from pUAST (Brand and Perrimon, 1993), containing 5 copies
of a GAL4 binding site and the Drosophila hsp70 minimal promoter was subcloned into BamHI site
of pBluescript containing an optimized GFP split with an intron (GG) (Zong et al., 2005). This
strategy generates a hybrid BglII/BamHI site in front of the GFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUAS-GG was a gift from Liqun Luo (Addgene plasmid # 24342 ; http://n2t.net/addgene:24342 ; RRID:Addgene_24342)
For your References section:The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990