pAC-GAL4
(Plasmid #24344)

• Depositing Lab: Liqun Luo
• Publication: Potter et al Volume 141, Issue 3, 536-548, 30 April 2010

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	24344	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pPac5c-PL
  (Search Vector Database)
• Backbone size w/o insert (bp): 6400
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GAL4
• Insert Size (bp): 2600

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: AC5

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry
• Reference:
  • pAC-GAL4 (GenBank)
• Articles Citing this Plasmid:
  • 9 References

Depositor Comments

GAL4 cDNA was PCR amplified using primers PR509
(CCCCGGATCCCAACatgaagctactgtcttctatcgaaca) and PR510
(CGGTTAACGCGGCCGCttactctttttttgggtttggtg), and a lab vector, pCA-GAL4 as the template. The
PCR product was cloned using BamHI and NotI into pPAC5C-PL.