Addgene - pAC-GAL4 Plasmid Data

Plasmid 24344: pAC-GAL4

Gene/insert name:
GAL4
Insert size:
2600
Vector backbone:
pPac5c-PL
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
6400
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
AC5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Supplemental document:
pAC-GAL4 (GenBank)
Principal Investigator:
Liqun Luo
Terms and Licenses:
MTA

Comments:

GAL4 cDNA was PCR amplified using primers PR509
(CCCCGGATCCCAACatgaagctactgtcttctatcgaaca) and PR510
(CGGTTAACGCGGCCGCttactctttttttgggtttggtg), and a lab vector, pCA-GAL4 as the template. The
PCR product was cloned using BamHI and NotI into pPAC5C-PL.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis Potter et al (Volume 141, Issue 3, 536-548, 30 April 2010 PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24344" in your Materials and Methods section.

Price: $65.00

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