Addgene - pENTR-Site1 Plasmid Data

Plasmid 24512: pENTR-Site1

Gene/insert name:
Split GFP-RFP chimera
Insert size:
3948
Species:
Aequorea victoria and Discosoma sp. reef coral
Vector backbone:
pDONR221
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Mouse Targeting, Cre/Lox
Backbone size w/o insert (bp):
2538
Cloning method:
Gateway Cloning
Cloning site 5':
attL1
Site destroyed during cloning:
No
Cloning site 3':
attL2
Site destroyed during cloning:
No
5' sequencing primer:
M13 forward List of Sequencing Primers
3' sequencing primer:
M13 reverse
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Kazuo Kinoshita
Terms and Licenses:
MTA
Clontech Limited Use Label License

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors. Uemura et al (PLoS One. 2010 . 5(3):e9846. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 24512" in your Materials and Methods section.