Addgene - CAGGS-losSTOPlox-ClaI flp-in Plasmid Data

Plasmid 21548: CAGGS-losSTOPlox-ClaI flp-in

Gene/insert name:
None
Insert size:
5400
Vector backbone:
Bluescript
(Search Vector Database)
Backbone manufacturer:
stratagene
Vector type:
Mammalian Expression, Mouse Targeting
Backbone size w/o insert (bp):
3000
Cloning site 5':
ClaI or NheI
Site destroyed during cloning:
No
5' sequencing primer:
M13 forward List of Sequencing Primers
3' sequencing primer:
M13 reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Selectable markers, other:
hygromycin after flp-in
Sequence: View sequences (2)
Principal Investigator:
Rudolf Jaenisch
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Beard et al (Genesis. 2006 Jan . 44(1):23-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21548" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

20730: Col1a-frt-hygro-pA